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semi wet transfer xcell ii mini blot module  (Thermo Fisher)


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    Thermo Fisher semi wet transfer xcell ii mini blot module
    Semi Wet Transfer Xcell Ii Mini Blot Module, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xcell+ii+blot+module+semi+wet+transfer/pm20970820-221-13-19
    Average 86 stars, based on 1 article reviews
    semi wet transfer xcell ii mini blot module - by Bioz Stars, 2026-09
    86/100 stars

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    Electrophoresis:

    Article Title: Expression of epithelial calcium transport system in rat cochlea and vestibular labyrinth
    Article Snippet: A colored marker mixture (#161-0375, Bio-Rad) was used for estimation of molecular weight. .. After electrophoresis, proteins were transferred onto a PVDF membrane (#162-0175, Bio-Rad) using the XCell II Blot Module semi-wet transfer unit (#EI0002, Invitrogen) at 24 V for 75 min using transfer buffer [25 mM Tris-Base, 192 mM glycine, 20% methanol, pH 8.3]). .. The membrane was then blocked with a blocking buffer (5% non-fat dry milk [#170-6404, Bio-Rad] in TBS [20 mM Tris-Base, 137 mM NaCl, pH 7.6] containing 0.05% Tween 20) for 1 hr at room temperature and then probed overnight at 4°C with 1:400 primary antibody (TRPV5, #CAT21A, Alpha Diagnostics, San Antonio, TX).

    Membrane:

    Article Title: Expression of epithelial calcium transport system in rat cochlea and vestibular labyrinth
    Article Snippet: A colored marker mixture (#161-0375, Bio-Rad) was used for estimation of molecular weight. .. After electrophoresis, proteins were transferred onto a PVDF membrane (#162-0175, Bio-Rad) using the XCell II Blot Module semi-wet transfer unit (#EI0002, Invitrogen) at 24 V for 75 min using transfer buffer [25 mM Tris-Base, 192 mM glycine, 20% methanol, pH 8.3]). .. The membrane was then blocked with a blocking buffer (5% non-fat dry milk [#170-6404, Bio-Rad] in TBS [20 mM Tris-Base, 137 mM NaCl, pH 7.6] containing 0.05% Tween 20) for 1 hr at room temperature and then probed overnight at 4°C with 1:400 primary antibody (TRPV5, #CAT21A, Alpha Diagnostics, San Antonio, TX).



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